The programmed temperature pushed the mixture from
the stable phase (below the solubility-saturation curve) at a starting
temperature of 80 ◦C to the metastable- (undercooling) and
unstable-(supercooling, above the supersaturation curve) phases
at final temperatures of 20, 25 and 30 ◦C. At a lower final temperature,
the concentration of DHSA (the solute) in the mixture
was higher than its solubility in comparison to that at a higher
final temperature and thus yielded more DHSA crystals.