Following incubation the MAb-coated PCR tubes were washed three times with 1× PBST, pH 7.4. The 50 _l of the clarified prepared extracts from suspected CiLV-C infected and healthy citrus leaf tissues were added to the G10 MAb captured individual tubes, incubated overnight at 4◦C, and followed the next day by washing three times with 1× PBST, pH 7.4 and then once with nuclease-free water