3.3. Biosensor operation
A syringe pump (KD Scientific) was used to apply positive
pressure at the inlet to generate fluid flow. The outlet was open
to the atmosphere. The connection between the syringe pump
and the device was made using PEEK tubing with 1/16 in. outer
diameter. Samples were loaded into the microfluidic channel
through inlet at a flow rate of approximately 10L/min. The
devicewas incubated at 20 ◦C for 2 hours. Following incubation,
the device was rinsed by pumping distilled, deionized water
through the flow chamber.