All blood samples were taken using a non-heparinised vacutainer and then incubated at ambient temperature for 2 h to allow clotting. Samples were centrifuged at 4300g to collect the sera which was stored at −20 ◦C until analysis.
All blood samples were taken using a non-heparinised vacutainer and then incubated at ambienttemperature for 2 h to allow clotting. Samples were centrifuged at 4300g to collect the sera which was stored at −20 ◦C untilanalysis.