In all chromatograms obtained for the investigated peppers, the main identified peaks of interest
corresponded to these two predominant capsaicinoids. On the other hand, it should be noticed that
another peak (c) corresponding to nordihydrocapsaicin is also observed in all chromatograms.
The ultraviolet absorption spectra corresponding to capsaicin and dihydrocapsaicin peaks were
recorded from the photodiode array detector; they are shown in Figures 3 and 4, respectively. For all
quantitative measurements, the UV detection wavelength was set at 222 nm, because it corresponds to
the maximum absorbance for both capsaicin and dihydrocapsaicin. On the other hand, the
chromatograms showed a complete separation between capsaicin and dihydrocapsaicin and no
interference with other capsaicinoid peaks.