Incubation was done at room temperature (28 ± 2ºC) for 48 hours. In the case of
temperature, the incubation was done at different temperatures (10, 20, 30, 40
and 50ºC).
Determination of growth:
Yeast growth was estimated by measuring the optical density at 540 nm
using Shimadzu UV-1601 spectrophotometer.