27PCR amplification was performed in a thermocycler (TC-512 Techne resistance, Burlington, NJ, USA) under thefollowing conditions: initial denaturation at 95◦C for 5 minfollowed by 30 cycles of 95◦C for 1 min, 56◦C for 1 min and72◦C for 1 min, and final extension at 72◦C for 5 min