This review discusses current information related to enzyme
product formulation for ruminants, and addresses the conditions necessary to ensure effective and consistent in vivo results of providing
feed enzymes to ruminants. Research has demonstrated that, in some cases, adding fibrolytic enzymes to dairy cow and
feedlot cattle diets improves cell wall digestion and, consequently, weight gain or milk production are enhanced. However, considerable
research is required to develop more effective enzyme products and to ensure consistency of responses in vivo. There is
a need to identify the key enzyme activities involved in the positive responses observed in vivo and these enzyme activities should
be assessed using a temperature and pH representative of the conditions in the rumen. However, to date, it has not been possible
to accurately evaluate exogenous enzymes based only on their biochemical characterization because the model substrates used do
not represent the complexity of plant cell wall material. In vitro techniques using feed substrates, buffer and ruminal fluid can be
used more reliably as a bioassay to predict in vivo response to exogenous enzymes, however, other factors, including under or
over-supplementation of enzyme activity, method of providing the enzyme product to the animal, composition of the diet, and the
target animals must also be considered
This review discusses current information related to enzymeproduct formulation for ruminants, and addresses the conditions necessary to ensure effective and consistent in vivo results of providingfeed enzymes to ruminants. Research has demonstrated that, in some cases, adding fibrolytic enzymes to dairy cow andfeedlot cattle diets improves cell wall digestion and, consequently, weight gain or milk production are enhanced. However, considerableresearch is required to develop more effective enzyme products and to ensure consistency of responses in vivo. There isa need to identify the key enzyme activities involved in the positive responses observed in vivo and these enzyme activities shouldbe assessed using a temperature and pH representative of the conditions in the rumen. However, to date, it has not been possibleto accurately evaluate exogenous enzymes based only on their biochemical characterization because the model substrates used donot represent the complexity of plant cell wall material. In vitro techniques using feed substrates, buffer and ruminal fluid can beused more reliably as a bioassay to predict in vivo response to exogenous enzymes, however, other factors, including under orover-supplementation of enzyme activity, method of providing the enzyme product to the animal, composition of the diet, and thetarget animals must also be considered
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