d-Glucaric acid was identified as a “top value-added chemical
from biomass” in a report by the department of energy of
U.S. [3]. Biological production of d-glucaric acid by microorganism
was mainly studied by Dr. Prather’s group in MIT [19,39,40].
E. coli was found to have the native route for the production
of d-glucaric acid from d-glucose. However, there were
more than 10 steps in this pathway, making it difficult to be