Effect of A. paniculata supplemented fish feeds against S. agalactiae
infection In vivo studies of the effect of A. paniculata supplemented fish feeds on
S. agalactiae infection of Nile tilapia were performed using groups of 10 uninfected
fish fed with fish feeds no. 1–12 separately for 5 days. On day 6, fish were infected with
S. agalactiae by intraperitoneal injection of 50 μl of bacterial suspension, at a dose
causing 50% mortality (LD50). The infected fish were reared for further 10 days with the
assigned test fish feed. Mortality, appearance, feeding response, and behavior of the fish
were observed daily.