K. marxianus CCA510, a non-conventional yeast in xylitol pro-duction, was able to grow and produce the polyol in the differentmedia evaluated, showing low inhibition by the inhibitory com-pounds present in the hydrolysate. The maximum yield of xylitolobtained by this microorganism was 12.73 g L−1using CABH-2hydrolysate and the nitrogen sources studied, in the studied con-centrations, showed no significant influence on the production ofxylitol by the yeast evaluated. It is concluded that the productionof xylitol from cashew apple bagasse hydrolysate using K. marxi-anus CCA510 is undoubtedly promising, since it does not requireany supplementation with minerals or other substances.