demonstrated the use of by HCWs in daily practice, as a source of nosocomial infections in hospitals. They also demonstrated the rate of routine cleaning of HCWs' MPs was 10.5% Nosocomial infection represents an important cause of morbidity and mortality for children admitted in the Pediatric Intensive Care Unit , the overall mortality attributable to NI has been estimated at 11% The aim of this study was to measure colonization by bacterial microorganism, between HCWs and PVs, in POPICU at Children’s Hospital, Ain Shams University in Cairo, Egypt.