A pilot-scale study of biohydrogen production was performed in a continuous flow anaerobic fermentation reactor (with an
available volume of 1.48 m3) for over 200 days. The hydrogen bio-producing reactor (HBR) system was operated under the
organic loading rates (OLR) of 3.11–85.57 kg COD/m3reactor/d (COD: chemical oxygen demand) with molasses as the substrate.
Both biogas and hydrogen yields increased with OLR at the range of 3.11–68.21 kgCOD/m3 d, but decreased at high OLR
(68.21–85.57 kg COD/m3reactor/d). The biogas was mainly composed of CO2 and H2 with the percentage of H2 ranging from
40% to 52% in biogas. A maximum hydrogen production rate of 5.57 m3H2/m3reactor/d, with a specific hydrogen production
rate of 0.75 m3H2/kg MLVSS/d, was obtained in the reactor. The hydrogen yield reached 26.13 mol/kg CODremoved within
OLR range of 35–55 kg COD/m3reactor/d. In addition, it was found that the hydrogen yield was affected by the presence of
ethanol and acetate in the liquid phase, and the maximum hydrogen production rate occurred while the ratio of ethanol to acetate
was close to 1, due to the adjustment of NAD+/(NADH+H+) through fermentation pathways. Ethanol-type fermentation was
favorable for hydrogen production.
2006 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
A pilot-scale study of biohydrogen production was performed in a continuous flow anaerobic fermentation reactor (with an
available volume of 1.48 m3) for over 200 days. The hydrogen bio-producing reactor (HBR) system was operated under the
organic loading rates (OLR) of 3.11–85.57 kg COD/m3reactor/d (COD: chemical oxygen demand) with molasses as the substrate.
Both biogas and hydrogen yields increased with OLR at the range of 3.11–68.21 kgCOD/m3 d, but decreased at high OLR
(68.21–85.57 kg COD/m3reactor/d). The biogas was mainly composed of CO2 and H2 with the percentage of H2 ranging from
40% to 52% in biogas. A maximum hydrogen production rate of 5.57 m3H2/m3reactor/d, with a specific hydrogen production
rate of 0.75 m3H2/kg MLVSS/d, was obtained in the reactor. The hydrogen yield reached 26.13 mol/kg CODremoved within
OLR range of 35–55 kg COD/m3reactor/d. In addition, it was found that the hydrogen yield was affected by the presence of
ethanol and acetate in the liquid phase, and the maximum hydrogen production rate occurred while the ratio of ethanol to acetate
was close to 1, due to the adjustment of NAD+/(NADH+H+) through fermentation pathways. Ethanol-type fermentation was
favorable for hydrogen production.
2006 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
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