Crude extract was dissolved in n-hexane. The total carotenoid content estimated as _-carotene was quantified spectrophotometrically at 450nm by using a five-point standard calibration curve using _-carotene (Sigma–Aldrich).
Crude extract was dissolved in n-hexane. The total carotenoid content estimated as _-carotene was quantified spectrophotometrically at 450nm by using a five-point standard calibration curve using _-carotene (Sigma–Aldrich).