In the present study, the DPPH radical quenching
activity of the extract was detected. The extract exhibited
maximum percentage inhibition of 124% at the
concentration of 150 μg/mL with an IC50 value of 15.5
μg/mL, in a concentration dependent manner (r2=
0.957, Figure 2). DPPH is relatively stable nitrogen
centered free radical that easily accepts an electron or
hydrogen radical to become a stable diamagnetic molecule,
which makes its use advantageous in evaluating
antioxidant activities. DPPH radicals react with
suitable reducing agents as a result of which the electrons
become paired off forming the corresponding
hydrazine38. Several studies reported direct correlation
between the antioxidant activity and polyphenolic
content of the plant extract which was also seen in
present investigation39.