2.2. Solid state fermentation
Germinated Palmyra palm seeds, after removal of palm sprout,were collected from Sivagangai district, Tamilnadu, India. The outer shell was removed and the kernel was carefully taken and then washed with distilled water to remove the impurities. The kernel was cut into pieces and solar-dried for a week to prevent microbial contamination. The carbon and nitrogen contents of Asian Palmyra palm kernel were determined by CHNS/O analyzer (Series II 2400, Perkin Elmer, USA) and found to be 33.5 wt% and 0.39 wt% respectively.