Among the 116 positive blood cultures analysed by the
array, 111 samples yielded identifications consistent with those
obtained by conventional methods. Among the five samples
with discrepant results, four were detected as multiple species
fungaemia (MSF) by the array, but were reported as single-species infection using conventional methods (Table 2). Conventional isolation and identification were repeated in these four
samples and the presence of additional yeast species, the same
as that detected by the array, was confirmed in all four cultures. The remaining discrepant sample was identified as containing Rhodotorula mucilaginosa by the array, but was reported
as containing R. glutinis using the Vitek YBC. To differentiate
R. mucilaginosa from R. glutinis, the supplementary nitrate
assimilation test, which requires an incubation period of 4 days
and is not routinely performed in the clinical laboratory [20],
was required. Identification of the discrepant isolate as R. mucilaginosa by the array was confirmed by sequence analysis of the amplified ITS region using the BLAST program (GenBank
accession no. EU781664). Representative hybridization results
are shown in Fig. 2
Among the 116 positive blood cultures analysed by thearray, 111 samples yielded identifications consistent with thoseobtained by conventional methods. Among the five sampleswith discrepant results, four were detected as multiple speciesfungaemia (MSF) by the array, but were reported as single-species infection using conventional methods (Table 2). Conventional isolation and identification were repeated in these foursamples and the presence of additional yeast species, the sameas that detected by the array, was confirmed in all four cultures. The remaining discrepant sample was identified as containing Rhodotorula mucilaginosa by the array, but was reportedas containing R. glutinis using the Vitek YBC. To differentiateR. mucilaginosa from R. glutinis, the supplementary nitrateassimilation test, which requires an incubation period of 4 daysand is not routinely performed in the clinical laboratory [20],was required. Identification of the discrepant isolate as R. mucilaginosa by the array was confirmed by sequence analysis of the amplified ITS region using the BLAST program (GenBankaccession no. EU781664). Representative hybridization resultsare shown in Fig. 2
การแปล กรุณารอสักครู่..
