Separation of anthocyanins was carried out with an HPLC system
equipped with an InertSustain C18 (5 lm, 250 4.6 mm ID)
column and a VIS 535 nm (LC800 UV) detector (Hitachi, Tokyo,
Japan), using an injection volume of 20 ll, and operated at room
temperature with a flow rate of 1 ml/min.