2. Materials and methods
2.1. Raw materials
Barnyard millet (cv. VL Madira 172) was procured from
Vivekananda Parvatiya Krishi Anusandhan Sansthan (VPKAS),
Almora. The grain was cleaned, dehulled, polished (1 min in rice
polisher) and pulverized into fine flour (Sieve Mesh No. 65 m). This
flour was packaged in air tight container till further use. The millet
contained 11.82 g/100 g moisture, 8.39 g/100 g protein, 2.21 g/100 g
crude fat, 1.35 g/100 g minerals, 76.23 g/100 g carbohydrates and
6.5 g/100 g crude fiber. The wet gluten content determination
indicated that it has no (0 g/100 g) gluten content. Commercial
refined wheat flour, sugar powder, eggs, full fat milk, shortening/
margarine and salt (Tata) were purchased from the local market of
Ludhiana. All the chemicals used in the present study were purchased
from S.D. Fine Chemicals Ltd. Mumbai, India.