Fig. 2. NAcy constituents of MGP were associated with decreased of adipocyte differentiation. (A) Protein expression of PPARγ, aP2, HDAC9, H3K9Ac and H3 by Western blot analysis
from mice fed with either LF, HF or HF+0.4% MGP for 15 weeks. Cultures of hASCs were differentiated and incubated with either Acy or NAcy fractions for 10 days. (B) TG accumulation
in 96 well culture plates was visualized by ORO staining (upper panel). Extracted ORO staining was quantified (OD 500 nm) (lower panel). (C), Bright-field images with ORO staining
for cultures differentiated with different doses of NAcy and Acy. (D) Adipogenic gene expression of PPARγ and aP2 by qPCR analysis. In panels B and D, values are presented as the
mean±S.E.M. Means not sharing a common superscript differ significantly (Pb.05) by one-way ANOVA with Tukey's multiple comparison test (aNbNc).