Figure 7: Cell-free GFP expression over time. Comparison of the commercial kit with the DiaMIX was performed using the self-synthesized Spy-GFP-10xHis. Relative fluorescence was measured every minute over 2 hours. As a negative control, the fluorescence of the respective mix without adding DNA was recorded. The experiment was performed in triplicates and the values normalized to air. A propagation of uncertainty was performed.