Phosphate solubilizing microorganisms (PSM) were analyzed in
Pikovskaya medium (Pikovskaya, 1948). In counting PSM, colonies
were identified by the clear zone observed around the colony. In
estimating phosphate solubilizing bacteria (PSB) was 30 mg/l of
cycloheximide was added to the Pikovskaya media to inhibit fungi
growth, and this was supplemented with 30 mg/l of streptomycin to
prevent the growth of bacteria in counting phosphate solubilizing
fungi (PSF).
Arbuscular mycorrhizal colonization (AMC) was quantifies by
counting arbuscules and vesicles in vetiver root following the
method ofKoske and Gemma (1989). Roots were separated from
soil by hand. Lateral roots were taken from each plot to determine VAM root infection. Roots stained with 0.05% Trypan blue
were mounted on a slide with lactophenol, a cover slip was
placed over the root segments, and then the roots were examined
under a microscope and the numbers of vesicles and arbuscules
counted