Continuous countercurrent chromatography has a significant potential
for the purification of plant-derived biopharmaceuticals because
continuous processing reduces the column sizes necessary for the
separation of plant extracts and therefore the associated time and
costs (Warikoo et al., 2012). This method has recently been used to isolate
proteins with anti-cancer activity from bitter melon (Momordica
charantia) achieving 86–94% purity