Methods: Ninety Charolais cows and heifers were entered into the program an average of 1.8
times. Thus, 164 animal cases were included. Animals were administered 10-12 μg of buserelin.
Seven days later animals without a corpus luteum (CL) were rejected (20.7%) while the remaining
130 cases with a CL were administered prostaglandin F2α, followed 48 h later with a second
injection of buserelin (8-10 μg). Fixed-AI was performed 16-20 hours after the last injection.