To evaluate the performance of different assembly methods in terms of SNP-calling, we analysed SNPs called using assemblies from the GAGE-B study (Magoc et al., 2013) for R. sphaeroides and M. abscessus (Dataset 1). Assemblies were available for eight most popular assembly methods (Abyss, Cabog, Mira, MaSuRCA, SGA, SoapDenovo, SPAdes and Velvet) with both HiSeq and MiSeq reads