The total phenolic content was determined using the FCR[15]. The
reaction mixture contained 100 μL of methanolic solution (1 mg/mL)
of extract, 0.5 mL of FCR, 1.5 mL of 20% (w/v) sodium carbonate
and 10 mL of distilled water. After 2h of reaction at ambient
temperature, the absorbance was measured at 765 nm and used to
calculate the phenolic contents, using gallic acid as a standard. Then
the total phenolic contents were expressed in term of gallic acid
equivalents (mg GAE/g dry extract).