Wemeasured
the integrated forms of WT NL-GFP and NL-GFP mut
DNA in CA2/Cas9, IN1/Cas9 and T5/Cas9 cells 2 days
after challenge. In this real-time PCR assay, we used a
primer set that specifically amplifies the LTR U3 region of
HIV-1, which is deleted in the self-inactivating lentiviral
vector used in this study.