At laboratory-scale. The working volume in a fed-batch culture at 5-
l-jar fermenter increased from 2.5 to 3 l by feeding glucose and glycerol solution
(where the percentage of glucose and glycerol 1.0% and 0.5%, respectively)
after 72 h. When the carbon source was depleted, the feeding solution of 100 ml
was added intermittently five times. The feeding solution was added by
peristaltic pump coupled with pH probe. The total added carbon source was
45 g in fed-batch culture. The colchicine and thiocolchicine were fed after 36 h
into the 5-l-jar fermenter containing B. megaterium ACBT03-M3. The total
amount of the substrates (colchicine and thiocolchicine) added to was 21 g.