A sand culture pot experiment was conducted under
naturally illuminated environmental conditions during
the rabi winter. Healthy seeds of two genotypes of
mustard (Brassica juncea L. Czern and Coss.) cv.
Chuutki (salt-sensitive) and cv. Radha (salt-tolerant)
were selected for the study. These genotypes were
emanated on the basis of results of our earlier experiment
(Siddiqui et al. 2009). The earthen pots of 30
cm diameter, lined with polythene bags (to avoid
contamination), were filled with sand. Before filling
the pots the sand was washed with acid, followed by
tap and distilled water. The pots were arranged in a
simple randomized design with a single factor and 4
replicates. Before sowing, seeds were surface sterilized
with 1% sodium hypochlorite solution for 10 min,
then vigorously rinsed with double distilled water
(DDW) and sown in sand filled pots supplied with
Raukura’s nutrient solution. After 2 weeks of sowing,
thinning was done and three healthy plants of uniform
size were maintained in each pot. When the plants
were at the stage of 2-3 true leaves, NaCl solution
was added to the pots with experimental Brassica plants
to attain the final concentration. A half dose of N was
applied basally at the time of sowing, and the remaining
half dose was given as a spray treatment after 1
week of NaCl treatment. The experimental pots were
irrigated daily with DDW (100-200 mL) to keep the
sand moist. The treatments were given as follows: (i)
0 mmol L-1 NaCl + 0 mg N kg-1 sand (NaCl0+ N0,
control), (ii) 90 mmol L-1 NaCl + 30 mg N kg-1 sand
(NaCl90+ N30), (iii) 90 mmol L-1 NaCl + 60 mg N kg-1
A sand culture pot experiment was conducted undernaturally illuminated environmental conditions duringthe rabi winter. Healthy seeds of two genotypes ofmustard (Brassica juncea L. Czern and Coss.) cv.Chuutki (salt-sensitive) and cv. Radha (salt-tolerant)were selected for the study. These genotypes wereemanated on the basis of results of our earlier experiment(Siddiqui et al. 2009). The earthen pots of 30cm diameter, lined with polythene bags (to avoidcontamination), were filled with sand. Before fillingthe pots the sand was washed with acid, followed bytap and distilled water. The pots were arranged in asimple randomized design with a single factor and 4replicates. Before sowing, seeds were surface sterilizedwith 1% sodium hypochlorite solution for 10 min,then vigorously rinsed with double distilled water(DDW) and sown in sand filled pots supplied withRaukura’s nutrient solution. After 2 weeks of sowing,thinning was done and three healthy plants of uniformsize were maintained in each pot. When the plantswere at the stage of 2-3 true leaves, NaCl solutionwas added to the pots with experimental Brassica plantsto attain the final concentration. A half dose of N wasapplied basally at the time of sowing, and the remaininghalf dose was given as a spray treatment after 1week of NaCl treatment. The experimental pots wereirrigated daily with DDW (100-200 mL) to keep thesand moist. The treatments were given as follows: (i)0 mmol L-1 NaCl + 0 mg N kg-1 sand (NaCl0+ N0,control), (ii) 90 mmol L-1 NaCl + 30 mg N kg-1 sand(NaCl90+ N30), (iii) 90 mmol L-1 NaCl + 60 mg N kg-1
การแปล กรุณารอสักครู่..
