The medium was autoclaved at 121 °C for 20 min. Seed medium (0.2% yeast extract, 0.2% mycological peptone, ASW 50%, pH 6.5) and production medium (1% yeast extract, 0.1% mycological peptone, ASW 50%, pH 6.5) was autoclaved followed by the addition of syringe filtered (0.2 μm) 0.5% glucose and 1% glucose, respectively.