The activity of the samples were measured at time t=0 and t=60 min.
The constant of the ouabaine-dependent outflow rate was obtained by
subtracting the total constant and ouabaine-independent constant.
The entire research, except for marking the concentration of zinc in
serum and the concentration of this element in lymphocytes, was performed
in the Isotopic Laboratory of Department and Clinic of Cardiology
Medical Academy in Wrocl⁄aw. The concentration of zinc in serum and the
concentration of zinc in lymphocytes were measured by using atomic
spectrophotometry with an ASS Perkin-Elmer Model 5000.
To evaluate the significance of statistical differences between tested
samples in the case of normal distribution, the Student’s t-test was used,
and in the case of variables not displaying normal distribution, the Kolmogorov-
Smirnov test was used. Calculations were made using Statistica
for Windows program, version 51G (edition 97).
The whole population was statistically worked out taking into consideration
the correlation between particular parameters and particular
subgroups of the population by differences between them.
RESULTS
Values of particular parameters (x ± SD) in tested groups of primary
arterial hypertension in comparison with the group of healthy people are
presented in the Table 2.
In the tested group of healthy patients, values of Zn-s and Zn-l were
higher than in the tested group of patients with arterial hypertension. Zns
correlated negatively with values of sRR, dRR, and IVSd. Similarly,
ERCt-Zn correlated negatively with values of sRR, dRR, and IVS and positively
with ARO. ARO in turn correlated negatively with values of sRR
and dRR, and ACE correlated positively with Ald-s.
Taking into consideration the possible pressure mechanism of zinc
impact described earlier, including the part of RAAS that takes place in regulation,
it might be thought that the correlations described above indicate
their part in the regulation of arterial pressure in physiological conditions.
In mild arterial hypertension, there is a decrease of Zn-s and Zn-l and
an increase of ERCt-Zn and also an insignificant increase of ERCos-Zn.
By using simple regression in the group of patients with mild arterial
hypertension, positive statistical correlations were displayed between
ERCt-Zn and ERCos-Zn (r = 0.73) and ERCt-Zn and Zn-s (r = 0.51). By
using multiple regression, significant dependency was displayed between
ERCt-Zn and ERCos-Zn positive, and Zn-s positive, and Zn-l negative,
and Ald-s positive and ACE negative (r = 0.88).
Dependency was also shown between Zn-s and ERCt-Zn positive,
and Zn-l negative and K-s positive (r = 0.76). Furthermore, a negative
dependency between Zn-l with ERCt-Zn and a positive dependency with
Zn-s (r = 0.89).
92 Tubek
Biological Trace Element Research Vol. 107, 2005
The activity of the samples were measured at time t=0 and t=60 min.The constant of the ouabaine-dependent outflow rate was obtained bysubtracting the total constant and ouabaine-independent constant.The entire research, except for marking the concentration of zinc inserum and the concentration of this element in lymphocytes, was performedin the Isotopic Laboratory of Department and Clinic of CardiologyMedical Academy in Wrocl⁄aw. The concentration of zinc in serum and theconcentration of zinc in lymphocytes were measured by using atomicspectrophotometry with an ASS Perkin-Elmer Model 5000.To evaluate the significance of statistical differences between testedsamples in the case of normal distribution, the Student’s t-test was used,and in the case of variables not displaying normal distribution, the Kolmogorov-Smirnov test was used. Calculations were made using Statisticafor Windows program, version 51G (edition 97).The whole population was statistically worked out taking into considerationthe correlation between particular parameters and particularsubgroups of the population by differences between them.RESULTSValues of particular parameters (x ± SD) in tested groups of primaryarterial hypertension in comparison with the group of healthy people arepresented in the Table 2.In the tested group of healthy patients, values of Zn-s and Zn-l werehigher than in the tested group of patients with arterial hypertension. Znscorrelated negatively with values of sRR, dRR, and IVSd. Similarly,ERCt-Zn correlated negatively with values of sRR, dRR, and IVS and positivelywith ARO. ARO in turn correlated negatively with values of sRRand dRR, and ACE correlated positively with Ald-s.Taking into consideration the possible pressure mechanism of zincimpact described earlier, including the part of RAAS that takes place in regulation,it might be thought that the correlations described above indicatetheir part in the regulation of arterial pressure in physiological conditions.In mild arterial hypertension, there is a decrease of Zn-s and Zn-l andan increase of ERCt-Zn and also an insignificant increase of ERCos-Zn.By using simple regression in the group of patients with mild arterialhypertension, positive statistical correlations were displayed betweenERCt-Zn and ERCos-Zn (r = 0.73) and ERCt-Zn and Zn-s (r = 0.51). Byusing multiple regression, significant dependency was displayed betweenERCt-Zn and ERCos-Zn positive, and Zn-s positive, and Zn-l negative,and Ald-s positive and ACE negative (r = 0.88).Dependency was also shown between Zn-s and ERCt-Zn positive,and Zn-l negative and K-s positive (r = 0.76). Furthermore, a negativedependency between Zn-l with ERCt-Zn and a positive dependency withZn-s (r = 0.89).92 TubekBiological Trace Element Research Vol. 107, 2005
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