2. Guava Leaf Extraction. The sample extraction by maceration. Weighed as much as 50 g of guava leaves, soaked in 150 mL of ethanol and acetone with a concentration of 30%, 50% and 70% for 24 hours and then filtered to obtain a filtrate. Treatment was for 3 days. Filtrate obtained together then evaporated to obtain ethanol and acetone extracts. The evaporated extract was cooled in a desiccator before further analysis.