The strain of Lentinus strigosus (Schwinitz) Fries was taken from the collection of fungi at INPA Institute. Mycelial fragments of fungus (stored in test tubes) were transferred to a Petri dish containing malt medium and incubated at 27 ºC until colonization by the fungus (primary matrix) that was used as a source ofinoculum for the secondary matrix . Mycelial disks, 9 mm in diameter, were removed from
the primary matrix and transferred to Petri dishes containing SDA medium (sawdust-dextrose-agar), prepared according to Sales-Campos (2008), named secondary matrix. "Spawn" is the source for inoculation of the cultivation substrate, considered here as a tertiary matrix.