Samples
(300 μL, in triplicate) were mixed with 1.5 mL of the 10 %
Folin-Ciocalteu reagent, followed by an addition of 1.2 mL of
7.5 % (w/v) sodium carbonate (Na2CO3) solution. The test
tubes were then left to stand for 30 min in the dark at room
temperature before the absorbance values were measured at
765 nm. The total phenolic content was expressed as mg gallic
acid equivalent per 100 g of sample (mg GAE/100 g)