To validate the idea of self-calibration, a tree-shaped paper
device is designed, which could assure the uniformity of micro
flows in different branches for multiplex concentration of analytes
on the paper. This tree-shaped paper device offers 7 assays,
which means a set of 7 data could be collected at the same time,
including the color intensity in both the standard solution and the
unknown concentration (Fig. 2). When developing reagent water
goes from stem to a wider one, its frontline spreads from the previous
straight line to a semi-circle. Consequently, in our design all
the branches start at the periphery of the same semi-circle, whose
center is in the middle of the stem’s top; all BSA dots, as well as BPB
dots, remain the same distance to the center. This could guarantee
equal fluid situation of water in each of the branches and therefore
ensures the accuracy of our results. Moreover, this device is made
merely by carefully cutting, without any complicated process, such
as lithography or building hydrophobic barriers.
To validate the idea of self-calibration, a tree-shaped paper
device is designed, which could assure the uniformity of micro
flows in different branches for multiplex concentration of analytes
on the paper. This tree-shaped paper device offers 7 assays,
which means a set of 7 data could be collected at the same time,
including the color intensity in both the standard solution and the
unknown concentration (Fig. 2). When developing reagent water
goes from stem to a wider one, its frontline spreads from the previous
straight line to a semi-circle. Consequently, in our design all
the branches start at the periphery of the same semi-circle, whose
center is in the middle of the stem’s top; all BSA dots, as well as BPB
dots, remain the same distance to the center. This could guarantee
equal fluid situation of water in each of the branches and therefore
ensures the accuracy of our results. Moreover, this device is made
merely by carefully cutting, without any complicated process, such
as lithography or building hydrophobic barriers.
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