1) Run blank solution (DI-water) and sugar standards, by setting the conditions as follow,
i. The operating flow rate of mobile phase 1.0 ml/min.
ii. The refractive index (RI) detector’s temperature is 30ºC.
iii. The mobile phase ratio of acetonitrile to 0.01M HCl is 84:16.
iv. The analysis sample volume is 50.00 ul.
v. The chromatographic column is the Alltech® Prevail™ amino group (5 um, Length 150 mm, I.D 4.6 mm).
vi. The operation time for blank solution and sugar standards are 20 and 50 min, respectively.
2) Analyze the chromatogram or retention time of the investigated standard solution by using the previous conditions.