All reagents, standards and solvents were of analytical grade. Coomassie brilliant blue, albumin bovine serum (P96%), phenol, glucose (P98%) and sodium carbonate were purchased from Sigma–Aldrich (Slovenia)
All reagents, standards and solvents were of analytical grade.Coomassie brilliant blue, albumin bovine serum (P96%), phenol,glucose (P98%) and sodium carbonate were purchased fromSigma–Aldrich (Slovenia)