Fig. 3 showed that the maximum predicted alcoholic degree can be achieved when AC, BT, and MC were 0.9&, 98 C, and 38%,respectively. The contour had the look of umbrella shape and the highest value of alcoholic degree was nearly in the center of contour graph. The variations of alcoholic degree with temperature were not consistent with the report of starch hydrolysis by Govindasamy et al. (1997). This might be because the most suitable temperature of amylase was 95-100 C and amylase activity could decrease beyond the suitable temperature range. The alcoholic degree also decreased when AC was beyond 0.9&, which was due to the inhibition of oligosaccharides to yeasts (Loureiro-Dias & Peinado, 1982).
degree also decreased when AC was beyond 0.9&, which was due to the inhibition of oligosaccharides to yeasts (Loureiro-Dias &Peinado, 1982).