A stock culture of a mated strain of
(Maassen ex Claussen) Olive and Spiltoir, originally
isolated from chalkbrood mummies received
from Ohio in 1990, was maintained in the
laboratory on potato dextrose agar (Difco) fortified
with extract (4 mg/ml; Difco) at
30°C. Subcultures were made every d.
For this study, small blocks of agar from the
stock culture containing both mycelia and
spores were transferred to fortified potato dextrose
broth plates (15 media per each 100 x
15 mm polystyrene Petri dish) and incubated at
30°C. Every d mycelia and spores were
removed from the broth with forceps, blotted on
paper towels to remove excess broth, and
stored refrigerated in ethanol (95%) until neede