Genomic DNA was extracted from each sample using
600 ll of lysis buffer (100 mM Tris–HCl pH 8.0, 100 mM
sodium EDTA, phosphate buffer pH 8.0, 1.5 M NaCl, 1%
CTAB), 10 ll lysozyme and 10 ll protinase K, followed by
mixing and incubating at 37 C for 30 min. After that 100 ll
of 20% SDS was added into the mixture and further incubated
at 65 C for 30 min.