3.2. In vitro biocontrol activity of living yeasts against A. carbonarius
On YPD agar only living cellswere able to significantly prevent path-
ogen development (Table 1). On YPD enriched with filtered or filtered
and subsequently sterilized culture broth, A. carbonarius was not
inhibited: in fact, when sterile culture broth from strains
L. thermotolerans 751 and C. friedrichii 778 was added to YPD agar,
A. carbonarius colony growth was significantly higher compared to the
control plates (Table 1).
On YES agar medium, the presence of living yeast cells prevented
A. carbonarius growth, while the pathogen was only slightly – albeit
significantly – inhibited by the filtered/autoclaved culture broth from