Banana waste is discarded due to the imperfection during grading process. Banana biomass can be
used as raw material to produce bioethanol. In this study, fermentation of banana waste was conducted
using Saccharomyces cerevisiae, Type II under anaerobic condition. Production of bioethanol was
determined and the effects of various operating conditions which included different temperatures,
shaking period, rotten and fresh banana fruit and saccharification method were observed. Overall, the
fermented banana fruit waste produced 4.1 to 7.1% bioethanol. The bioethanol yield from mixture of
rotten banana fruit increased with increase fermentation period. It is also increased with yeast
concentration, using 35% of water at 35°C. The optimum shaking hours for fermentation was 6 h at pH
5.8. Combination of enzyme (pectinase and cellulase) produced higher bioethanol than enzyme alone.
Viscosity and acid value of the produced bioethanol followed the ASTM (American Standard for Testing
Materials) and EN (European Norms) standards. Fermented banana treated with mixture of enzymes
was the best method used for higher bioethanol production. The results showed that, utilization of
mixture (skin and pulp) of rotten fruit was more suitable for bioethanol production as renewable energy
which could reduce the cost of the initial process. In addition, it did not compete with the consumer
food supply and could avoid the overloaded waste for compose, as well as could be used as fuel in the
normal petrol engine. In addition to that, energy could be produced from waste banana fruit as
environmental recycling process.