Cereals, such as wheat and barley, contain significant amount of non-starch polysaccharides which are poorly digestible but play a role as available energy resource in current farming systems. Additive enzymes have positive effects both on digestibility and environmental pollution by lowering the extraction of phosphorous and nitrogen in the manure. Discovery of new bacterial strains that have enzymes with unique and improved properties remains a very important way of advancing the field. A collaborative project (YEM-EN) has been initiated to produce and develop commonly used imported poultry feed additives-phytase, xylanase, beta-glucanase enzymes- produced by bacteria, and search for availability of these enzymes in poultry nutrition. In this project, fromdifferent local ecosystems 162 phytase, 130 xylanase, 114 beta-glucanase producing microorganisms were isolated, identified and stored as culture collection. All isolates were screened in liquid culture media and three isolates were selected as the best enzyme producers. Identification of these strains by 16S rDNA showed that these strains belong to the species Bacillus subtilis, Bacillus pumilus and Bacillus amyloliquefaciens. Enzyme production processes of the three enzymes were optimized for pilot scale. Moreover, these enzymes were granulated and then tested in diet for bioavailability in broilers and laying hens. Simultaneously, related genes were cloned, expressed and characterized. The national production and development of technology for industrial feed enzymes will result in the reduction of annual expenditure and the maintenance of independency of poultry feed compounders in Turkey.