Label the swab tube with sufficient detail.
• Remove the swab stick from the sterile wrapping.
• Moisten the tip, if required, by immersing it in a tube containing dilution liquid.
• Press the tip of the tube against the inside wall of the tube to remove excess fluid.
• Select the area to be swabbed and apply a suitably sized often 100cm2
• Press the tip of the swab onto the surface and streak in two directions at right angles within the template whilst rotating the swab stick between thumb and forefinger.) steriletemplate.
• Put the swab back in the tube with the dilution material and aseptically break or cut off the stick.
• Clean the sampled area and the templates with alcohol wipes.
Incubation petri plate for 3 days at 22.5ºC