In the second experiment, T. atroviride SC1 needed one week longer to colonize the bark, due to the greater quantity of bark present. Pieces of wood (7 mm diameter, 50 mm length) from 1-year-old apple shoots were autoclaved twice at 121 C for 30 min. The sterilized pieces of wood were then placed in Petri dishes (90 mm diameter)containing MEA and were inoculated with plugs of A. gallica(3 3 3 mm) taken from a colony grown for 3weeks on MEA. Five inoculum plugs were placed in each dish