used a wide range of explants (anthers,
ovaries, peduncles, petioles, zygotic embryos) of C. persicum cv.
‘Rosamunde’ to produce callus. They used 1/2 MS basal medium
containing 1.0 mg l−1 2,4-D with or without 10% coconut milk. All
explants produced slow-growing callus. Best growth was observed
in the dark and callus cultures were pale or brownish and hard
regardless of the PGR applied. When cultured alone with auxin,
callus growth was poor and the explants died soon. Karam and
Al-Majathoub (2000b) collected leaf discs, petioles, petals, and
peduncles from wild Jordanian cyclamen plants for culture. All
explants were cultured on MS medium supplemented with different
concentrations of BA or TDZ.