Pollen storage at −20◦C showed almost similar trend as observed in case of −4◦C storage.
The only difference noticed was the percentage viability as it was high in all three cultivars at all observation dates compared to pollen storage at −4◦C.
In ‘Sensation’, pollen viability after 24 weeks of storage at −20◦C showed decrease of 79.23% in vitro germination (Fig. 1C), 79.04% by FDA test (Fig. 1G) and 62.15% by acetocarmine test (Fig. 1K) compared to fresh pollen viability.
Similarly, in ‘Tommy Atkins’ and ‘Janardan Pasand’, the decrease in pollen viability after 24 weeks of storage at −20◦C was 74.94 and 76.57%, respectively as depicted by in vitro germination test (Fig. 1C).
Fluorescein diacetate and acetocarmine tests also confirmed decrease in viability of pollen stored at −20◦C.
Decrease in pollen viability of ‘Tommy Atkins’ was 75.90% as determined by FDA test (Fig. 1G) and 61.62% by acetocarmine test (Fig. 1K).
In ‘Janardan Pasand’, the decrease was 78.07% (FDA test) and 60.46% (acetocarmine test) as compared to fresh pollen viability (Fig. 1G and K).
Pollen storage at −20◦C showed almost similar trend as observed in case of −4◦C storage. The only difference noticed was the percentage viability as it was high in all three cultivars at all observation dates compared to pollen storage at −4◦C. In ‘Sensation’, pollen viability after 24 weeks of storage at −20◦C showed decrease of 79.23% in vitro germination (Fig. 1C), 79.04% by FDA test (Fig. 1G) and 62.15% by acetocarmine test (Fig. 1K) compared to fresh pollen viability. Similarly, in ‘Tommy Atkins’ and ‘Janardan Pasand’, the decrease in pollen viability after 24 weeks of storage at −20◦C was 74.94 and 76.57%, respectively as depicted by in vitro germination test (Fig. 1C). Fluorescein diacetate and acetocarmine tests also confirmed decrease in viability of pollen stored at −20◦C. Decrease in pollen viability of ‘Tommy Atkins’ was 75.90% as determined by FDA test (Fig. 1G) and 61.62% by acetocarmine test (Fig. 1K). In ‘Janardan Pasand’, the decrease was 78.07% (FDA test) and 60.46% (acetocarmine test) as compared to fresh pollen viability (Fig. 1G and K).
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