FHB experiments. All head blight experiments were conducted
in a glasshouse, as previously described (24). Barley Lux
plants were grown in a heated glasshouse (22°C, 16-h day, and
12°C, 8-h night; maximum temperature of 27°C). At midanthesis,
heads (two per plant) were sprayed to runoff with either 20 µM
epiBL or water (control plants). At 24 h post hormone application,
the same heads were treated with either Tween 20 or
F. culmorum conidia (105
ml–1), as previously described (24).
Visual disease symptoms were recorded at growth stage (GS) 80
(start of dough development) based on the percentage of bleached
spikelets per head. Treated heads were harvested at GS 99 (50).
The number of seed per head and total seed yield (in grams) per
head were recorded. Each treatment combination was applied to
16 plants (two heads per plant) and the experiment was conducted
twice (February to April 2012 and March to May 2012) in independent
glasshouses in a randomized layout.
FHB experiments. All head blight experiments were conductedin a glasshouse, as previously described (24). Barley Luxplants were grown in a heated glasshouse (22°C, 16-h day, and12°C, 8-h night; maximum temperature of 27°C). At midanthesis,heads (two per plant) were sprayed to runoff with either 20 µMepiBL or water (control plants). At 24 h post hormone application,the same heads were treated with either Tween 20 orF. culmorum conidia (105 ml–1), as previously described (24).Visual disease symptoms were recorded at growth stage (GS) 80(start of dough development) based on the percentage of bleachedspikelets per head. Treated heads were harvested at GS 99 (50).The number of seed per head and total seed yield (in grams) perhead were recorded. Each treatment combination was applied to16 plants (two heads per plant) and the experiment was conductedtwice (February to April 2012 and March to May 2012) in independentglasshouses in a randomized layout.
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