Many studies have been published in this research field. Previous studies have confirmed that the lipid content in some microalgae can be enhanced by applying various cultivation conditions
such as nitrogen or phosphate starvation (Khozin-Goldberg and Cohen, 2006), and high light intensity (Cheirsilp and Torpee,2012). Among these methods, using carbon and nitrogen sources
are known to affect the metabolism of lipids and fatty acids in various microalgae.