Fig. 1. Lipid analysis of isolated lipid droplets. A. Thin layer chromatography plate showing lipid fractions from isolated lipid droplets (LD, lane 1) or whole worm (lane 2), demonstrating
enrichment of triacylglycerols (TAG) in the isolated lipid droplets. Other lipid classes in lipid droplets include phosphatidylethanolamine (PE) and phosphatidylcholine (PC). B. The ratio
of triacylglycerols/phospholipids (TAG/PL) is increased in daf-2 wholeworms and daf-2 lipid droplets compared to wild type. C. The ratio of phosphatidylcholine/phosphtidylethanolamine
(PC/PE) is not different in daf-2mutants compared towild type. D. Very little cholesterol (Chol) or cholesterol esters (Chol ester) are present in C. elegans lipid droplets. Standards are shown
in lanes 1–3 and chloroform extracts of C. elegans lipid droplets are shown in lanes 4–5. Other lipid classes include triacylglycerols (TAG), free fatty acids (FFA) and phospholipids (PL).